• Immunohistochemistry analysis of paraffin-embedded rat kidney using MTCO2 Rabbit polyclonal antibody (STJ113171) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using MTCO2 Rabbit polyclonal antibody (STJ113171) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MTCO2 antibody (STJ113171) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MT-CO2 antibody (101-200) (STJ113171)

SKU:
STJ113171

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTCO2 (101-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MT-CO2
Gene ID: 4513
Uniprot ID: COX2_HUMAN
Immunogen Region: 101-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 101-200 of mouse MTCO2 (NP_904331.1).
Immunogen Sequence: GHQWYWSYEYTDYEDLCFDS YMIPTNDLKPGELRLLEVDN RVVLPMELPIRMLISSEDVL HSWAVPSLGLKTDAIPGRLN QATVTSNRPGLFYGQCSEIC
Function Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Protein Name Cytochrome C Oxidase Subunit 2
Cytochrome C Oxidase Polypeptide Ii
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome C Oxidase Subunit 2 antibody
Anti-Cytochrome C Oxidase Polypeptide Ii antibody
Anti-MT-CO2 antibody
Anti-COII antibody
Anti-COX2 antibody
Anti-COXII antibody
Anti-MTCO2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance