• Immunohistochemistry analysis of paraffin-embedded mouse lung using GDF-8/Myostatin antibody (STJ28993) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of HeLa, using GDF-8/Myostatin Rabbit polyclonal antibody (STJ28993) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MSTN antibody (267-375) (STJ28993)

SKU:
STJ28993

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MSTN (267-375) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSTN
Gene ID: 2660
Uniprot ID: GDF8_HUMAN
Immunogen Region: 267-375
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 267-375 of human GDF-8/Myostatin (NP_005250.1).
Immunogen Sequence: DFGLDCDEHSTESRCCRYPL TVDFEAFGWDWIIAPKRYKA NYCSGECEFVFLQKYPHTHL VHQANPRGSAGPCCTPTKMS PINMLYFNGKEQIIYGKIPA MVVDRCGCS
Post Translational Modifications Synthesized as large precursor molecule that undergoes proteolytic cleavage to generate an N-terminal propeptide and a disulfide linked C-terminal dimer, which is the biologically active molecule. The circulating form consists of a latent complex of the C-terminal dimer and other proteins, including its propeptide, which maintain the C-terminal dimer in a latent, inactive state. Ligand activation requires additional cleavage of the prodomain by a tolloid-like metalloproteinase.
Function Acts specifically as a negative regulator of skeletal muscle growth.
Protein Name Growth/Differentiation Factor 8
Gdf-8
Myostatin
Database Links Reactome: R-HSA-9617828
Cellular Localisation Secreted
Alternative Antibody Names Anti-Growth/Differentiation Factor 8 antibody
Anti-Gdf-8 antibody
Anti-Myostatin antibody
Anti-MSTN antibody
Anti-GDF8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance