Anti-MSH6 antibody (900-1000 aa) [ABT-MSH6] (STJ196844)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenSynthesized peptide derived from the human MutS Protein Homolog 6 (MSH6) at the amino acid range 900-1000
General Information
| Short Description | Mouse monoclonal anti-DNA mismatch repair protein Msh6 (900-1000 aa) for use in IHC, WB and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB/IF |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ABT-MSH6 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | IHC-P 1:100-500WB 1:500-1000IF 1:500-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MSH6 |
| Gene ID | 2956 |
| Uniprot ID | MSH6_HUMAN |
| Immunogen | Synthesized peptide derived from the human MutS Protein Homolog 6 (MSH6) at the amino acid range 900-1000 |
| Immunogen Region | 900-1000 aa |
| Specificity | This antibody detects endogenous levels of human MutS Protein Homolog 6 (MSH6). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin sect |
Additional Info
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. |
| Protein Name | Dna Mismatch Repair Protein Msh6Hmsh6G/T Mismatch-Binding ProteinGtbpGtmbpMuts Protein Homolog 6Muts-Alpha 160 Kda SubunitP160 |
| Database Links | Reactome: R-HSA-5358565Reactome: R-HSA-5632928Reactome: R-HSA-5632968 |
| Cellular Localisation | NucleusChromosomeAssociates With H3k36me3 Via Its Pwwp Domain |
| Alternative Antibody Names | Anti-Dna Mismatch Repair Protein Msh6 antibodyAnti-Hmsh6 antibodyAnti-G/T Mismatch-Binding Protein antibodyAnti-Gtbp antibodyAnti-Gtmbp antibodyAnti-Muts Protein Homolog 6 antibodyAnti-Muts-Alpha 160 Kda Subunit antibodyAnti-P160 antibodyAnti-MSH6 antibodyAnti-GTBP antibody |
Information sourced from Uniprot.org