| Host: |
Mouse |
| Applications: |
IHC-P |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-MSH6 is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
44 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide |
| Dilution Range: |
IHC 1:25 – 1:100 |
| Storage Instruction: |
Store at 2-8°C upon receipt. |
| Post Translational Modifications | The N-terminus is blocked. Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway. |
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. |
| Protein Name | Dna Mismatch Repair Protein Msh6Hmsh6G/T Mismatch-Binding ProteinGtbpGtmbpMuts Protein Homolog 6Muts-Alpha 160 Kda SubunitP160 |
| Database Links | Reactome: R-HSA-5358565Reactome: R-HSA-5632928Reactome: R-HSA-5632968 |
| Cellular Localisation | NucleusChromosomeAssociates With H3k36me3 Via Its Pwwp Domain |
| Alternative Antibody Names | Anti-Dna Mismatch Repair Protein Msh6 antibodyAnti-Hmsh6 antibodyAnti-G/T Mismatch-Binding Protein antibodyAnti-Gtbp antibodyAnti-Gtmbp antibodyAnti-Muts Protein Homolog 6 antibodyAnti-Muts-Alpha 160 Kda Subunit antibodyAnti-P160 antibodyAnti-MSH6 antibodyAnti-GTBP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
