![Western blot analysis of lysates from wild type (WT) and MSH2 Rabbit polyclonal antibody knockout (KO) HeLa cells, using [KO Validated] MSH2 Rabbit polyclonal antibody (STJ11104374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177018/368207/STJ11104374_2__31134.1713133714.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MSH2 Rabbit polyclonal antibody knockout (KO) HeLa cells, using [KO Validated] MSH2 Rabbit polyclonal antibody (STJ11104374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from A-549 cells, using [KO Validated] MSH2 Rabbit polyclonal antibody (STJ11104374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177018/368208/STJ11104374_3__34752.1713133714.jpg?c=1 "Western blot analysis of lysates from A-549 cells, using [KO Validated] MSH2 Rabbit polyclonal antibody (STJ11104374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-MSH2 antibody (1-300) (STJ11104374)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Dna Mismatch Repair Protein Msh2 (1-300) antibody for use in WB, IF, ICC, IP and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MSH2 |
| Gene ID | 4436 |
| Uniprot ID | MSH2_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MAVQPKETLQLESAAEVGFV RFFQGMPEKPTTTVRLFDRG DFYTAHGEDALLAAREVFKT QGVIKYMGPAGAKNLQSVVL SKMNFESFVKDLLLVRQYRV EVYKNRAGNKASKENDWYLA YKASPGNLSQFEDILFGNND MSASIGVVGVKMSAVDGQRQ VGVGYVDSIQRKLGLCEFPD NDQFSNLEALLIQIGPKECV LPGGETAGDMGKLRQIIQRG GILITERKKADFSTKDIYQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway. Sequentially deacetylated and polyubiquitinated by HDAC6, leading to MSH2 degradation. |
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. |
| Protein Name | Dna Mismatch Repair Protein Msh2Hmsh2Muts Protein Homolog 2 |
| Database Links | Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5632927Reactome: R-HSA-5632928Reactome: R-HSA-5632968Reactome: R-HSA-6796648 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Dna Mismatch Repair Protein Msh2 antibodyAnti-Hmsh2 antibodyAnti-Muts Protein Homolog 2 antibodyAnti-MSH2 antibody |
Information sourced from Uniprot.org