• Anti-MS4A1 antibody (100-200) [S5MR] (STJ11102185)
  • Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and Daudi cells (right) were surface-stained with CD20 Rabbit monoclonal antibody (STJ11102185, 10 Mu g/mL orange line) or ABflo® 647 Rabbit IgG isotype control ( 10 Mu g/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).
  • Confocal imaging of Daudi and Jurkat (Negative control) cells using CD20 Rabbit monoclonal antibody (STJ11102185, dilution 1:1000) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD20 Rabbit monoclonal antibody (STJ11102185) at dilution of 1:8000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human anaplastic large cell lymphoma using CD20 Rabbit monoclonal antibody (STJ11102185) at dilution of 1:8000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using CD20 Rabbit monoclonal antibody (STJ11102185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : HeLaExposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using CD20 Rabbit monoclonal antibody (STJ11102185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-MS4A1 antibody (100-200) [S5MR] (STJ11102185)

SKU:
STJ11102185

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD20 (100-200) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:2000-1:10000
IF/ICC 1:500-1:1000
FC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MS4A1
Gene ID: 931
Uniprot ID: CD20_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD20 (NP_068769.2).
Immunogen Sequence: LLAATEKNSRKCLVKGKMIM NSLSLFAAISGMILSIMDIL NIKISHFLKMESLNFIRAHT PYINIYNCEPANPSEKNSPS TQYCYSIQSLFLGILSVMLI F
Tissue Specificity Expressed on B-cells.
Post Translational Modifications Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.
Function B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.
Protein Name B-Lymphocyte Antigen Cd20
B-Lymphocyte Surface Antigen B1
Bp35
Leukocyte Surface Antigen Leu-16
Membrane-Spanning 4-Domains Subfamily A Member 1
Cd Antigen Cd20
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Lipid-Anchor
Constitutively Associated With Membrane Rafts
Alternative Antibody Names Anti-B-Lymphocyte Antigen Cd20 antibody
Anti-B-Lymphocyte Surface Antigen B1 antibody
Anti-Bp35 antibody
Anti-Leukocyte Surface Antigen Leu-16 antibody
Anti-Membrane-Spanning 4-Domains Subfamily A Member 1 antibody
Anti-Cd Antigen Cd20 antibody
Anti-MS4A1 antibody
Anti-CD20 antibody

Information sourced from Uniprot.org

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