| Short Description : | Rabbit polyclonal Small Ribosomal Subunit Protein Us7m (40-140) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MRPS7 |
| Gene ID: | 51081 |
| Uniprot ID: | RT07_HUMAN |
| Immunogen Region: | 40-140 |
| Immunogen Sequence: | EFKDPLIDKEYYRKPVEELT EEEKYVRELKKTQLIKAAPA GKTSSVFEDPVISKFTNMMM IGGNKVLARSLMIQTLEAVK RKQFEKYHAASAEEQATIER N |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 40-140 of human MRPS7 (NP_057055.2). |
| Protein Name | Small Ribosomal Subunit Protein Us7m28s Ribosomal Protein S7 - MitochondrialMrp-S7S7mtBmrp-27aBmrp27a |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Small Ribosomal Subunit Protein Us7m antibodyAnti-28s Ribosomal Protein S7 - Mitochondrial antibodyAnti-Mrp-S7 antibodyAnti-S7mt antibodyAnti-Bmrp-27a antibodyAnti-Bmrp27a antibodyAnti-MRPS7 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KO Validated] MRPS7 Rabbit polyclonal antibody (STJ11105463) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178107/370287/STJ11105463_1__49689.1713136221.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] MRPS7 Rabbit polyclonal antibody (STJ11105463) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 5s.")
![Western blot analysis of lysates from wild type (WT) and MRPS7 knockdown (KD) HeLa cells using [KO Validated] MRPS7 Rabbit polyclonal antibody (STJ11105463) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178107/370288/STJ11105463_2__45619.1713136222.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPS7 knockdown (KD) HeLa cells using [KO Validated] MRPS7 Rabbit polyclonal antibody (STJ11105463) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 5s.")
![Western blot analysis of various lysates using [KO Validated] MRPS7 Rabbit polyclonal antibody (STJ11105463) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178107/370287/STJ11105463_1__49689.1713136221.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] MRPS7 Rabbit polyclonal antibody (STJ11105463) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 5s.")
![Immunohistochemistry analysis of MRPL21 in paraffin-embedded human colon tissue using [KD Validated] MRPL21 Rabbit polyclonal antibody (STJ11105472) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/178116/370302/STJ11105472_1__52208.1713136235.jpg?c=1 "Immunohistochemistry analysis of MRPL21 in paraffin-embedded human colon tissue using [KD Validated] MRPL21 Rabbit polyclonal antibody (STJ11105472) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
