• Immunohistochemistry analysis of MRPL54 in paraffin-embedded Human colon using MRPL54 Rabbit polyclonal antibody (STJ117156) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MRPL54 Rabbit polyclonal antibody (STJ117156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MRPL54 antibody (1-138) (STJ117156)

SKU:
STJ117156

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MRPL54 (1-138) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL54
Gene ID: 116541
Uniprot ID: RM54_HUMAN
Immunogen Region: 1-138
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human MRPL54 (NP_758455.1).
Immunogen Sequence: MATKRLFGATRTWAGWGAWE LLNPATSGRLLARDYAKKPV MKGAKSGKGAVTSEALKDPD VCTDPVQLTTYAMGVNIYKE GQDVPLKPDAEYPEWLFEMN LGPPKTLEELDPESREYWRR LRKQNIWRHNRLSKNKRL
Protein Name Large Ribosomal Subunit Protein Ml54
39s Ribosomal Protein L54 - Mitochondrial
L54mt
Mrp-L54
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ml54 antibody
Anti-39s Ribosomal Protein L54 - Mitochondrial antibody
Anti-L54mt antibody
Anti-Mrp-L54 antibody
Anti-MRPL54 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance