• Immunofluorescence analysis of U-2 OS cells using MRPL23 Rabbit polyclonal antibody (STJ118852) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MRPL23 in paraffin-embedded human colon carcinoma using MRPL23 Rabbit polyclonal antibody (STJ118852) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MRPL23 in paraffin-embedded human thyroid cancer using MRPL23 Rabbit polyclonal antibody (STJ118852) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MRPL23 Rabbit polyclonal antibody (STJ118852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MRPL23 antibody (1-153) (STJ118852)

SKU:
STJ118852

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MRPL23 (1-153) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL23
Gene ID: 6150
Uniprot ID: RM23_HUMAN
Immunogen Region: 1-153
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human MRPL23 (NP_066957.3).
Immunogen Sequence: MARNVVYPLYRLGGPQLRVF RTNFFIQLVRPGVAQPEDTV QFRIPMEMTRVDLRNYLEGI YNVPVAAVRTRVQHGSNKRR DHRNVRIKKPDYKVAYVQLA HGQTFTFPDLFPEKDESPEG SAADDLYSMLEEERQQRQSS DPRRGGVPSWFGL
Protein Name Large Ribosomal Subunit Protein Ul23m
39s Ribosomal Protein L23 - Mitochondrial
L23mt
Mrp-L23
L23 Mitochondrial-Related Protein
Ribosomal Protein L23-Like
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ul23m antibody
Anti-39s Ribosomal Protein L23 - Mitochondrial antibody
Anti-L23mt antibody
Anti-Mrp-L23 antibody
Anti-L23 Mitochondrial-Related Protein antibody
Anti-Ribosomal Protein L23-Like antibody
Anti-MRPL23 antibody
Anti-L23MRP antibody
Anti-RPL23L antibody

Information sourced from Uniprot.org

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