• Immunohistochemistry analysis of MRPL21 in paraffin-embedded human colon tissue using [KD Validated] MRPL21 Rabbit polyclonal antibody (STJ11105472) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using MRPL21 Rabbit polyclonal antibody (STJ11105472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of lysates from wild type (WT) and MRPL21 knockdown (KD) 293T cells using MRPL21 Rabbit polyclonal antibody (STJ11105472) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MRPL21 antibody (40-140) (STJ11105472)

SKU:
STJ11105472

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Large Ribosomal Subunit Protein Bl21m (40-140) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL21
Gene ID: 219927
Uniprot ID: RM21_HUMAN
Immunogen Region: 40-140
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 40-140 of human MRPL21 (NP_852615.1).
Immunogen Sequence: NSQSTSYLPGYVPKTSLSSP PWPEVVLPDPVEETRHHAEV VKKVNEMIVTGQYGRLFAVV HFASRQWKVTSEDLILIGNE LDLACGERIRLEKVLLVGAD N
Protein Name Large Ribosomal Subunit Protein Bl21m
39s Ribosomal Protein L21 - Mitochondrial
L21mt
Mrp-L21
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Bl21m antibody
Anti-39s Ribosomal Protein L21 - Mitochondrial antibody
Anti-L21mt antibody
Anti-Mrp-L21 antibody
Anti-MRPL21 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance