| Short Description : | Rabbit polyclonal Large Ribosomal Subunit Protein Bl21m (40-140) antibody for use in WB, IHC-P and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MRPL21 |
| Gene ID: | 219927 |
| Uniprot ID: | RM21_HUMAN |
| Immunogen Region: | 40-140 |
| Immunogen Sequence: | NSQSTSYLPGYVPKTSLSSP PWPEVVLPDPVEETRHHAEV VKKVNEMIVTGQYGRLFAVV HFASRQWKVTSEDLILIGNE LDLACGERIRLEKVLLVGAD N |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 40-140 of human MRPL21 (NP_852615.1). |
| Protein Name | Large Ribosomal Subunit Protein Bl21m39s Ribosomal Protein L21 - MitochondrialL21mtMrp-L21 |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Bl21m antibodyAnti-39s Ribosomal Protein L21 - Mitochondrial antibodyAnti-L21mt antibodyAnti-Mrp-L21 antibodyAnti-MRPL21 antibody |
Information sourced from Uniprot.org
![Immunohistochemistry analysis of MRPL21 in paraffin-embedded human colon tissue using [KD Validated] MRPL21 Rabbit polyclonal antibody (STJ11105472) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178116/370302/STJ11105472_1__52208.1713136235.jpg?c=1 "Immunohistochemistry analysis of MRPL21 in paraffin-embedded human colon tissue using [KD Validated] MRPL21 Rabbit polyclonal antibody (STJ11105472) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of MRPL21 in paraffin-embedded human colon tissue using [KD Validated] MRPL21 Rabbit polyclonal antibody (STJ11105472) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178116/370302/STJ11105472_1__52208.1713136235.jpg?c=1 "Immunohistochemistry analysis of MRPL21 in paraffin-embedded human colon tissue using [KD Validated] MRPL21 Rabbit polyclonal antibody (STJ11105472) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Western blot analysis of various lysates using [KO Validated] MRPS7 Rabbit polyclonal antibody (STJ11105463) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/178107/370287/STJ11105463_1__49689.1713136221.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] MRPS7 Rabbit polyclonal antibody (STJ11105463) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 5s.")
