![Western blot analysis of lysates from wild type (WT) and MPG knockout (KO) HeLa cells, using [KO Validated] MPG Rabbit polyclonal antibody (STJ11100043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89115/357567/STJ11100043_1__91960.1713121694.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MPG knockout (KO) HeLa cells, using [KO Validated] MPG Rabbit polyclonal antibody (STJ11100043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and MPG knockout (KO) HeLa cells, using [KO Validated] MPG Rabbit polyclonal antibody (STJ11100043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89115/357567/STJ11100043_1__91960.1713121694.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MPG knockout (KO) HeLa cells, using [KO Validated] MPG Rabbit polyclonal antibody (STJ11100043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
Anti-MPG antibody (1-293) (STJ11100043)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MPG (1-293) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MPG |
| Gene ID | 4350 |
| Uniprot ID | 3MG_HUMAN |
| Immunogen Region | 1-293 |
| Immunogen Sequence | MPARSGAQFCRRMGQKKQRP ARAGQPHSSSDAAQAPAEQP HSSSDAAQAPCPRERCLGPP TTPGPYRSIYFSSPKGHLTR LGLEFFDQPAVPLARAFLGQ VLVRRLPNGTELRGRIVETE AYLGPEDEAAHSRGGRQTPR NRGMFMKPGTLYVYIIYGMY FCMNISSQGDGACVLLRALE PLEGLETMRQLRSTLRKGTA SRVLKDRELCSGPSKLCQAL AINKSFDQRDLAQDEAVWL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1). |
Additional Info
| Function | Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. |
| Protein Name | Dna-3-Methyladenine Glycosylase3-Alkyladenine Dna Glycosylase3-Methyladenine Dna GlycosidaseAdpgN-Methylpurine-Dna Glycosylase |
| Database Links | Reactome: R-HSA-110330Reactome: R-HSA-110331Reactome: R-HSA-110357 |
| Cellular Localisation | CytoplasmMitochondrion MatrixMitochondrion NucleoidNucleus |
| Alternative Antibody Names | Anti-Dna-3-Methyladenine Glycosylase antibodyAnti-3-Alkyladenine Dna Glycosylase antibodyAnti-3-Methyladenine Dna Glycosidase antibodyAnti-Adpg antibodyAnti-N-Methylpurine-Dna Glycosylase antibodyAnti-MPG antibodyAnti-AAG antibodyAnti-ANPG antibodyAnti-MID1 antibody |
Information sourced from Uniprot.org