![Anti-Monocyte Antigen antibody [IPO-M6] (STJ16100825)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149542/302392/STJ16100825_1__38257.1646940584.jpg?c=1 "Anti-Monocyte Antigen antibody [IPO-M6] (STJ16100825)")
![Anti-Monocyte Antigen antibody [IPO-M6] (STJ16100825)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/149542/302392/STJ16100825_1__38257.1646940584.jpg?c=1 "Anti-Monocyte Antigen antibody [IPO-M6] (STJ16100825)")
Anti-Monocyte Antigen antibody [IPO-M6] (STJ16100825)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenA BALB/c mouse was immunized with human cell line HL-60. Fusion partner: P3-X63-Ag8.653.
General Information
| Short Description | Mouse monoclonal anti-Monocyte antigen for use in FC, IHC and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IHC/IP |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | IPO-M6 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow cytometry (0.5-1.0 µg/million cells in 0.1 ml). Immunohistology (1-2 µg/ml for 30 min at RT). Immunoprecipitation (1-2 µg/million cells in 1ml anti mouse coated Sepharose-4B suspension). |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Immunogen | A BALB/c mouse was immunized with human cell line HL-60. Fusion partner: P3-X63-Ag8.653. |
Additional Info
| Background | IPO-M6 reacts with human leukemia cell line HL-60 and immuno-precipitates two proteins with MW of 48 and 52 kDa. IPO-M6 does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4. IPO-M6 can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification) , AMMonL (M4) and hairy cells leukemia are IPO-M6 positive. The antigen, defined by IPO-M6, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages are also positive. Malignant cells from patients with AML (M1 and M2) , T-ALL, B ALL are IPO-M6 negative. |
Information sourced from Uniprot.org