• Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-R8 Rabbit polyclonal antibody (STJ24012). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MonoMethyl-Histone H3-R8 in paraffin-embedded mouse testis using MonoMethyl-Histone H3-R8 Rabbit polyclonal antibody (STJ24012) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MonoMethyl-Histone H3-R8 in paraffin-embedded human breast cancer using MonoMethyl-Histone H3-R8 Rabbit polyclonal antibody (STJ24012) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MonoMethyl-Histone H3-R8 in paraffin-embedded rat heart using MonoMethyl-Histone H3-R8 Rabbit polyclonal antibody (STJ24012) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-R8 antibody (STJ24012) at 1:1000 dilution.
  • Western blot analysis of various lysates using MonoMethyl-Histone H3-R8 Rabbit polyclonal antibody (STJ24012) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Mono-Methyl-Histone H3-R8 antibody (STJ24012)

SKU:
STJ24012

$286.88 - $677.03
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mono-Methyl-Histone H3-R8 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:20-1:100
ChIP-seq 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic monomethylated peptide around R8 of human histone H3 (NP_003520.1).
Immunogen Sequence: TARKS
Background This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.

Information sourced from Uniprot.org

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