• Immunohistochemistry analysis of MOK in paraffin-embedded human liver using MOK Rabbit polyclonal antibody (STJ25285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MOK in paraffin-embedded rat heart using MOK Rabbit polyclonal antibody (STJ25285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MOK in paraffin-embedded mouse kidney using MOK Rabbit polyclonal antibody (STJ25285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from mouse liver, using MOK Rabbit polyclonal antibody (STJ25285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-MOK antibody (1-230) (STJ25285)

SKU:
STJ25285

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MOK (1-230) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MOK
Gene ID: 5891
Uniprot ID: MOK_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MOK (NP_055041.1).
Immunogen Sequence: MKNYKAIGKIGEGTFSEVMK MQSLRDGNYYACKQMKQRFE SIEQVNNLREIQALRRLNPH PNILMLHEVVFDRKSGSLAL ICELMDMNIYELIRGRRYPL SEKKIMHYMYQLCKSLDHIH RNGIFHRDVKPENILIKQDV LKLGDFGSCRSVYSKQPYTE YISTRWYRAPECLLTDGFYT YKMDLWSAGCVFYEIASLQP LFPGVNELDQISKIHDVIGT PAQKILTKFK
Tissue Specificity Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.
Post Translational Modifications Autophosphorylated.
Function Able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner.
Protein Name Mapk/Mak/Mrk Overlapping Kinase
Mok Protein Kinase
Renal Tumor Antigen 1
Rage-1
Cellular Localisation Cytoplasm
Cell Projection
Cilium
Nucleus
Alternative Antibody Names Anti-Mapk/Mak/Mrk Overlapping Kinase antibody
Anti-Mok Protein Kinase antibody
Anti-Renal Tumor Antigen 1 antibody
Anti-Rage-1 antibody
Anti-MOK antibody
Anti-RAGE antibody
Anti-RAGE1 antibody

Information sourced from Uniprot.org

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