• Immunohistochemistry analysis of MOB4 in paraffin-embedded human oophoroma using MOB4 Rabbit polyclonal antibody (STJ26745) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MOB4 in paraffin-embedded rat ovary using MOB4 Rabbit polyclonal antibody (STJ26745) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MOB4 Rabbit polyclonal antibody (STJ26745) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MOB4 antibody (1-225) (STJ26745)

SKU:
STJ26745

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MOB4 (1-225) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MOB4
Gene ID: 25843
Uniprot ID: PHOCN_HUMAN
Immunogen Region: 1-225
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human MOB4 (NP_056202.2).
Immunogen Sequence: MVMAEGTAVLRRNRPGTKAQ DFYNWPDESFDEMDSTLAVQ QYIQQNIRADCSNIDKILEP PEGQDEGVWKYEHLRQFCLE LNGLAVKLQSECHPDTCTQM TATEQWIFLCAAHKTPKECP AIDYTRHTLDGAACLLNSNK YFPSRVSIKESSVAKLGSVC RRIYRIFSHAYFHHRQIFDE YENETFLCHRFTKFVMKYNL MSKDNLIVPILEEEVQNSVS GESEA
Post Translational Modifications Phosphorylated on serine residues.
Function May play a role in membrane trafficking, specifically in membrane budding reactions.
Protein Name Mob-Like Protein Phocein
2c4d
Class Ii Mmob1
Mob1 Homolog 3
Mob3
Mps One Binder Kinase Activator-Like 3
Preimplantation Protein 3
Cellular Localisation Cytoplasm
Perinuclear Region
Membrane
Peripheral Membrane Protein
Golgi Apparatus
Golgi Stack Membrane
In A Perinuclear Punctate Pattern
Associated With Membranes And The Golgi Stacks
Alternative Antibody Names Anti-Mob-Like Protein Phocein antibody
Anti-2c4d antibody
Anti-Class Ii Mmob1 antibody
Anti-Mob1 Homolog 3 antibody
Anti-Mob3 antibody
Anti-Mps One Binder Kinase Activator-Like 3 antibody
Anti-Preimplantation Protein 3 antibody
Anti-MOB4 antibody
Anti-MOB3 antibody
Anti-MOBKL3 antibody
Anti-PHOCN antibody
Anti-PREI3 antibody
Anti-CGI-95 antibody

Information sourced from Uniprot.org

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