• Western blot analysis of extracts of various cell lines, using MMP20 antibody (STJ111833) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using MMP20 Rabbit polyclonal antibody (STJ111833) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-MMP20 antibody (108-310) (STJ111833)

SKU:
STJ111833

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP20 (108-310) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP20
Gene ID: 9313
Uniprot ID: MMP20_HUMAN
Immunogen Region: 108-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 108-310 of human MMP20 (NP_004762.2).
Immunogen Sequence: YRLFPGEPKWKKNTLTYRIS KYTPSMSSVEVDKAVEMALQ AWSSAVPLSFVRINSGEADI MISFENGDHGDSYPFDGPRG TLAHAFAPGEGLGGDTHFDN AEKWTMGTNGFNLFTVAAHE FGHALGLAHSTDPSALMYPT YKYKNPYGFHLPKDDVKGIQ ALYGPRKVFLGKPTLPHAPH HKPSIPDLCDSSSSFDAVTM LGK
Tissue Specificity Expressed specifically in the enamel organ.
Post Translational Modifications Autoactivates at least at the 107-Asn-|-Tyr-108 site.
Function Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Cleaves aggrecan at the '360-Asn-|-Phe-361' site.
Protein Name Matrix Metalloproteinase-20
Mmp-20
Enamel Metalloproteinase
Enamelysin
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-2022090
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Matrix Metalloproteinase-20 antibody
Anti-Mmp-20 antibody
Anti-Enamel Metalloproteinase antibody
Anti-Enamelysin antibody
Anti-MMP20 antibody

Information sourced from Uniprot.org

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