• Western blot analysis of extracts of various cell lines, using MMP14/MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using MMP14/MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224, 2 Mu g/mL, orange line) or ABflo® 488 rabbit IgG isotype control (2 Mu g/mL, blue line) , followed by Alexa Fluor® 488 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224, 2 Mu g/mL, orange line) or ABflo® 488 rabbit IgG isotype control (2 Mu g/mL, blue line) , followed by Alexa Fluor® 488 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224, 2 Mu g/mL, orange line) or ABflo® 647 rabbit IgG isotype control (2 Mu g/mL, blue line) , followed by Alexa Fluor® 647 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224, 2 Mu g/mL, orange line) or ABflo® 647 rabbit IgG isotype control (2 Mu g/mL, blue line) , followed by Alexa Fluor® 647 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).

Anti-MMP14 antibody (100-200) [S4MR] (STJ11101224)

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STJ11101224

$197.78 - $863.33
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Host: Rabbit
Applications: WB/FC/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MT1-MMP (100-200) is suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
FC 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP14
Gene ID: 4323
Uniprot ID: MMP14_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MMP14/MMP14/MT1-MMP (P50281).
Immunogen Sequence: GAEIKANVRRKRYAIQGLKW QHNEITFCIQNYTPKVGEYA TYEAIRKAFRVWESATPLRF REVPYAYIREGHEKQADIMI FFAEGFHGDSTPFDGEGGFL A
Tissue Specificity Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Post Translational Modifications The precursor is cleaved by a furin endopeptidase. Tyrosine phosphorylated by PKDCC/VLK.
Function Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.
Protein Name Matrix Metalloproteinase-14
Mmp-14
Mmp-X1
Membrane-Type Matrix Metalloproteinase 1
Mt-Mmp 1
Mtmmp1
Membrane-Type-1 Matrix Metalloproteinase
Mt1-Mmp
Mt1mmp
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Melanosome
Cytoplasm
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Forms A Complex With Bst2 And Localizes To The Cytoplasm
Alternative Antibody Names Anti-Matrix Metalloproteinase-14 antibody
Anti-Mmp-14 antibody
Anti-Mmp-X1 antibody
Anti-Membrane-Type Matrix Metalloproteinase 1 antibody
Anti-Mt-Mmp 1 antibody
Anti-Mtmmp1 antibody
Anti-Membrane-Type-1 Matrix Metalloproteinase antibody
Anti-Mt1-Mmp antibody
Anti-Mt1mmp antibody
Anti-MMP14 antibody

Information sourced from Uniprot.org

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