• Western blot analysis of extracts of various cell lines, using MMP14/MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using MMP14/MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224, 2 Mu g/mL, orange line) or ABflo® 488 rabbit IgG isotype control (2 Mu g/mL, blue line) , followed by Alexa Fluor® 488 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224, 2 Mu g/mL, orange line) or ABflo® 488 rabbit IgG isotype control (2 Mu g/mL, blue line) , followed by Alexa Fluor® 488 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224, 2 Mu g/mL, orange line) or ABflo® 647 rabbit IgG isotype control (2 Mu g/mL, blue line) , followed by Alexa Fluor® 647 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP rabbit monoclonal antibody (STJ11101224, 2 Mu g/mL, orange line) or ABflo® 647 rabbit IgG isotype control (2 Mu g/mL, blue line) , followed by Alexa Fluor® 647 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).

Anti-MMP14 antibody (100-200) [S4MR] (STJ11101224)

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STJ11101224

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/FC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P: 1:50-1:200
FC: 1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP14
Gene ID: 4323
Uniprot ID: MMP14_HUMAN
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MMP14/MMP14/MT1-MMP (P50281).
Immunogen Sequence: GAEIKANVRRKRYAIQGLKW QHNEITFCIQNYTPKVGEYA TYEAIRKAFRVWESATPLRF REVPYAYIREGHEKQADIMI FFAEGFHGDSTPFDGEGGFL A
Tissue Specificity Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Post Translational Modifications The precursor is cleaved by a furin endopeptidase. Tyrosine phosphorylated by PKDCC/VLK.
Function Endopeptidase that degrades various components of the extracellular matrix such as collagen. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a regulator of Notch signaling by mediating cleavage and inhibition of DLL1. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis. Acts as a negative regulator of the GDF15-GFRAL aversive response by mediating cleavage and inactivation of GFRAL.
Protein Name Matrix Metalloproteinase-14
Mmp-14
Mmp-X1
Membrane-Type Matrix Metalloproteinase 1
Mt-Mmp 1
Mtmmp1
Membrane-Type-1 Matrix Metalloproteinase
Mt1-Mmp
Mt1mmp
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-9839383
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Melanosome
Cytoplasm
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Forms A Complex With Bst2 And Localizes To The Cytoplasm
Alternative Antibody Names Anti-Matrix Metalloproteinase-14 antibody
Anti-Mmp-14 antibody
Anti-Mmp-X1 antibody
Anti-Membrane-Type Matrix Metalloproteinase 1 antibody
Anti-Mt-Mmp 1 antibody
Anti-Mtmmp1 antibody
Anti-Membrane-Type-1 Matrix Metalloproteinase antibody
Anti-Mt1-Mmp antibody
Anti-Mt1mmp antibody
Anti-MMP14 antibody

Information sourced from Uniprot.org

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