• Western blot analysis of extracts of various cell lines, using MLLT3/AF9 antibody (STJ11104692) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-MLLT3 antibody (1-150) [S4692RM] (STJ11104692)

SKU:
STJ11104692

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Protein Af-9 (1-150) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4692RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MLLT3
Gene ID: 4300
Uniprot ID: AF9_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human MLLT3/AF9 (NP_004520.2).
Immunogen Sequence: MASSCAVQVKLELGHRAQVR KKPTVEGFTHDWMVFVRGPE HSNIQHFVEKVVFHLHESFP RPKRVCKDPPYKVEESGYAG FILPIEVYFKNKEEPRKVRF DYDLFLHLEGHPPVNHLRCE KLTFNNPTEDFRRKLLKAGG DPNRSIHTSS
Tissue Specificity Enriched in undifferentiated hematopoietic stem cells in fetal liver, cord blood and bone marrow.
Function Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones. Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 'Lys-79' methylation (H3K79me). Plays a key role in hematopoietic stem cell (HSC) maintenance by preserving, rather than confering, HSC stemness. Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L.
Protein Name Protein Af-9
All1-Fused Gene From Chromosome 9 Protein
Myeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 Protein
Yeats Domain-Containing Protein 3
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-674695
Reactome: R-HSA-75955
Cellular Localisation Nucleus
Chromosome
Colocalizes With Acylated Histone H3
Colocalizes With Histone H3 Crotonylated At 'Lys-18' (H3k18cr)
Alternative Antibody Names Anti-Protein Af-9 antibody
Anti-All1-Fused Gene From Chromosome 9 Protein antibody
Anti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 Protein antibody
Anti-Yeats Domain-Containing Protein 3 antibody
Anti-MLLT3 antibody
Anti-AF9 antibody
Anti-YEATS3 antibody

Information sourced from Uniprot.org

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