Anti-MKRN3 antibody (237-287) (STJA0006396)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human MKRN3 AA range: 237-287
General Information
| Short Description | Rabbit polyclonal anti-Probable E3 ubiquitin-protein ligase makorin-3 (237-287) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MKRN3 |
| Gene ID | 7681 |
| Uniprot ID | MKRN3_HUMAN |
| Immunogen | Synthesized peptide derived from human MKRN3 AA range: 237-287 |
| Immunogen Region | 237-287 |
| Specificity | This antibody detects endogenous levels of human MKRN3 |
Additional Info
| Function | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Acts as a key developmental timer that helps ensure puberty begins at the appropriate age, by inhibiting premature activation of the reproductive hormone cascade. Epigenetically regulates GNRH1 transcription by disrupting the binding of methyl-DNA binding protein 3/MBD3 to the promoter of GNRH1. Mechanistically, mediates the non-proteolytic ubiquitination of MBD3 at multiple sites with 'Lys27' ubiquitin linkages and thereby regulates the methylation status of the genome, including GNRH1 promoter. Modulates the stability and translation of GNRH1 mRNA by mediating the non-proteolytic ubiquitination of PABP family members PABPC1, PABPC3 and PABPC4 at multiple sites. Also participates in the maintenance of genomic and epigenomic stability by regulating the abundance of APEX2 via 'Lys-48'-linked ubiquitination. |
| Protein Name | E3 Ubiquitin-Protein Ligase Makorin-3Ring Finger Protein 63Ring-Type E3 Ubiquitin Transferase Makorin-3Zinc Finger Protein 127 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Makorin-3 antibodyAnti-Ring Finger Protein 63 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Makorin-3 antibodyAnti-Zinc Finger Protein 127 antibodyAnti-MKRN3 antibodyAnti-D15S9 antibodyAnti-RNF63 antibodyAnti-ZNF127 antibody |
Information sourced from Uniprot.org