Anti-MKNK2 antibody (Internal) (STJ94173)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the Internal region of human Mnk2.
STJ94173
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General Information

Short DescriptionRabbit polyclonal anti-MAP kinase-interacting serine/threonine-protein kinase 2 (Internal) for use in WB, ELISA and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA/IHC
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
IHC-p 1:50-300
ELISA 2000-20000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMKNK2
Gene ID2872
Uniprot IDMKNK2_HUMAN
ImmunogenSynthesized peptide derived from the Internal region of human Mnk2.
Immunogen RegionInternal
SpecificityMnk2 Polyclonal Antibody detects endogenous levels of Mnk2 protein.

Additional Info

Tissue Specificity Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.
Post Translational Modifications Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase. Phosphorylated upon arsenic trioxide As(2)O(3) treatment. Phosphorylated by MAPK1/ERK2, MAPK11 and MAPK14. Dephosphorylated by PP2A.
Function Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Isoform 1 displays a high basal kinase activity, but isoform 2 exhibits a very low kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As(2)O(3)-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal.
Protein Name Map Kinase-Interacting Serine/Threonine-Protein Kinase 2
Map Kinase Signal-Integrating Kinase 2
Mapk Signal-Integrating Kinase 2
Mnk2
Database Links
Cellular Localisation Isoform 2: Nucleus
Pml Body
Isoform 1: Cytoplasm
Alternative Antibody Names Anti-Map Kinase-Interacting Serine/Threonine-Protein Kinase 2 antibody
Anti-Map Kinase Signal-Integrating Kinase 2 antibody
Anti-Mapk Signal-Integrating Kinase 2 antibody
Anti-Mnk2 antibody
Anti-MKNK2 antibody
Anti-GPRK7 antibody
Anti-MNK2 antibody

Information sourced from Uniprot.org

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