Anti-MKNK1 antibody (374-465) (STJ11100383)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MKNK1 (374-465) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MKNK1 |
| Gene ID | 8569 |
| Uniprot ID | MKNK1_HUMAN |
| Immunogen Region | 374-465 |
| Immunogen Sequence | VQGQAPEKGLPTPQVLQRNS STMDLTLFAAEAIALNRQLS QHEENELAEEPEALADGLCS MKLSPPCKSRLARRRALAQA GRGEDRSPPTAL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 374-465 of human MKNK1 (NP_003675.2). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1. |
| Function | May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. |
| Protein Name | Map Kinase-Interacting Serine/Threonine-Protein Kinase 1Map Kinase Signal-Integrating Kinase 1Mapk Signal-Integrating Kinase 1Mnk1 |
| Database Links | Reactome: R-HSA-1295596 |
| Cellular Localisation | Isoform 2: CytoplasmIsoform 3: CytoplasmNucleus |
| Alternative Antibody Names | Anti-Map Kinase-Interacting Serine/Threonine-Protein Kinase 1 antibodyAnti-Map Kinase Signal-Integrating Kinase 1 antibodyAnti-Mapk Signal-Integrating Kinase 1 antibodyAnti-Mnk1 antibodyAnti-MKNK1 antibodyAnti-MNK1 antibody |
Information sourced from Uniprot.org