| Host: | Goat |
| Applications: | Pep-ELISA/WB-Trf/IP/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-MK5/MAPKAPK5 (Internal) is suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labe |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | MAPKAPK5 |
| Gene ID: | 8550 |
| Uniprot ID: | MAPK5_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_003659.2; NP_620777.1 |
| Immunogen Sequence: | STEALDNVLPSAQ |
| Post Translational Modifications | Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4.which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated. |
| Function | Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. |
| Protein Name | Map Kinase-Activated Protein Kinase 5 Mapk-Activated Protein Kinase 5 Mapkap Kinase 5 Mapkap-K5 Mapkapk-5 Mk-5 Mk5 P38-Regulated/Activated Protein Kinase Prak |
| Database Links | Reactome: R-HSA-2559580 Reactome: R-HSA-5687128 Reactome: R-HSA-6804756 |
| Cellular Localisation | Cytoplasm Nucleus Translocates To The Cytoplasm Following Phosphorylation And Activation Interaction With Erk3/Mapk6 Or Erk4/Mapk4 And Phosphorylation At Thr-182 Activates The Protein Kinase Activity Followed By Translocation To The Cytoplasm Phosphorylation By Pka/Prkaca At Ser-115 Also Induces Nuclear Export |
| Alternative Antibody Names | Anti-Map Kinase-Activated Protein Kinase 5 antibody Anti-Mapk-Activated Protein Kinase 5 antibody Anti-Mapkap Kinase 5 antibody Anti-Mapkap-K5 antibody Anti-Mapkapk-5 antibody Anti-Mk-5 antibody Anti-Mk5 antibody Anti-P38-Regulated/Activated Protein Kinase antibody Anti-Prak antibody Anti-MAPKAPK5 antibody Anti-PRAK antibody |
Information sourced from Uniprot.org