Anti-MK5/MAPKAPK5 antibody (192-206) (STJ72941)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-MK5/MAPKAPK5 (192-206) is suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB-Trf/IP/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11928 labe |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | MAPKAPK5 |
| Gene ID | 8550 |
| Uniprot ID | MAPK5_HUMAN |
| Accession Number | NP_003659.2; NP_620777.1 |
| Immunogen Region | 192-206 |
| Immunogen Sequence | PQVLEAQRRHQKEKS |
Additional Info
| Post Translational Modifications | Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4.which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated. |
| Function | Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. |
| Protein Name | Map Kinase-Activated Protein Kinase 5Mapk-Activated Protein Kinase 5Mapkap Kinase 5Mapkap-K5Mapkapk-5Mk-5Mk5P38-Regulated/Activated Protein KinasePrak |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-5687128Reactome: R-HSA-6804756 |
| Cellular Localisation | CytoplasmNucleusTranslocates To The Cytoplasm Following Phosphorylation And ActivationInteraction With Erk3/Mapk6 Or Erk4/Mapk4 And Phosphorylation At Thr-182Activates The Protein Kinase ActivityFollowed By Translocation To The CytoplasmPhosphorylation By Pka/Prkaca At Ser-115 Also Induces Nuclear Export |
| Alternative Antibody Names | Anti-Map Kinase-Activated Protein Kinase 5 antibodyAnti-Mapk-Activated Protein Kinase 5 antibodyAnti-Mapkap Kinase 5 antibodyAnti-Mapkap-K5 antibodyAnti-Mapkapk-5 antibodyAnti-Mk-5 antibodyAnti-Mk5 antibodyAnti-P38-Regulated/Activated Protein Kinase antibodyAnti-Prak antibodyAnti-MAPKAPK5 antibodyAnti-PRAK antibody |
Information sourced from Uniprot.org