Anti-Mitofusin 1 antibody [R05-3A8] (STJA0035269)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of rat Mitofusin 1
General Information
| Short Description | Rabbit monoclonal anti-Mitofusin 1 for use in WB and IP in Mouse, Rat and Hamster samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Rabbit |
| Reactivity | Mouse/Rat/Hamster |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-3A8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Mfn1 |
| Gene ID | 192647 |
| Uniprot ID | MFN1_RAT |
| Immunogen | A synthetic peptide of rat Mitofusin 1 |
Additional Info
| Tissue Specificity | Ubiquitous. In brain, it is expressed at weaker level than MFN2, while it is expressed at a higher level than MFN2 in heart and testis. Expressed at high level in elongating spermatids of seminiferous tubules. |
| Post Translational Modifications | Ubiquitinated by non-degradative ubiquitin by PRKN. Deubiquitination by USP30 inhibits mitochondrial fusion. Ubiquitinated by MARCHF5. When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN. |
| Function | Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity. |
| Protein Name | Mitofusin-1Mitochondrial Transmembrane Gtpase Fzo1bTransmembrane Gtpase Mfn1 |
| Database Links | Reactome: R-RNO-5205685Reactome: -RNO-9013419Reactome: -RNO-983231 |
| Cellular Localisation | Mitochondrion Outer MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Mitofusin-1 antibodyAnti-Mitochondrial Transmembrane Gtpase Fzo1b antibodyAnti-Transmembrane Gtpase Mfn1 antibodyAnti-Mfn1 antibodyAnti-Fzo1b antibody |
Information sourced from Uniprot.org