• Immunofluorescence – anti-Miranda antibody in fly cells at 1:100 dilution; InsGAL4 UASCD8 GFP wandering L3; cells were fixed with 4% of PFA
  • Anti-Miranda antibody at 1:1,000 dilution; protein at 40 ng per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-Miranda antibody in fly cells at 1:100 dilution; InsGAL4 UASCD8 GFP wandering L3; cells were fixed with 4% of PFA

Anti-Miranda antibody (220 aa N-Term) (STJ140173)

SKU:
STJ140173

£135.50 - £393.50
Current Stock:
Host: Goat
Applications: WB/IF
Reactivity: D.melanogaster
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Miranda (220 aa N-Term) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: Epitope affinity purified
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:2000
IF 1:50-1:500
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Immunogen Region: 220 aa N-Term
Specificity: Detects endogenous levels of Miranda protein by immunofluorescence.
Immunogen: Recombinant peptide derived from within residues 220 aa to the N-terminus of Drosophila melanogaster Miranda produced in E. coli.
Immunogen Sequence: MSFSKAKLKRFNDVDVAICG SPAASNSSAGSAGSATPTAS SAAAAPPTVQPERKEQIEKF FKDAVRFASSSKEAKEFAIP KEDKKSKGLRLFRTPSLPQR LRFRPTPSHTDTATGSGSGA STAASTPLHSAATTPVKEAK SASRLKGKEALQYEIRHKNE LIESQLSQLDVLRRHVDQLK EAEAKLREEHELATSKTDRL IEALTSENLSHKALNE
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance