• Immunohistochemistry analysis of MIER1 in paraffin-embedded Mouse kidney using MIER1 Rabbit polyclonal antibody (STJ11100434) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MIER1 Rabbit polyclonal antibody (STJ11100434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MIER1 antibody (110-160) (STJ11100434)

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STJ11100434

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MIER1 (110-160) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MIER1
Gene ID: 57708
Uniprot ID: MIER1_HUMAN
Immunogen Region: 110-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 110-160 of human MIER1 (Q8N108).
Immunogen Sequence: SGENKEENIKDSSGQEDETQ SSNDDPSQSVASQDAQEIIR PRRCKYFDTNS
Tissue Specificity Ubiquitously expressed, but at very low levels. However, consistent level of expression are observed in heart, testis, thyroid, ovary and adrenal gland. Transcripts are up-regulated in breast carcinoma cell lines and tumor.
Function Transcriptional repressor regulating the expression of a number of genes including SP1 target genes. Probably functions through recruitment of HDAC1 a histone deacetylase involved in chromatin silencing.
Protein Name Mesoderm Induction Early Response Protein 1
Early Response 1
Er1
Mi-Er1
Hmi-Er1
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Nucleus
Isoform 3: Nucleus
Isoform 4: Cytoplasm
Isoform 5: Cytoplasm
Isoform 6: Cytoplasm
Alternative Antibody Names Anti-Mesoderm Induction Early Response Protein 1 antibody
Anti-Early Response 1 antibody
Anti-Er1 antibody
Anti-Mi-Er1 antibody
Anti-Hmi-Er1 antibody
Anti-MIER1 antibody
Anti-KIAA1610 antibody

Information sourced from Uniprot.org

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