Anti-MID2/TRIM1 antibody (C-Term) (STJ70313)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70313-100
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General Information

Short DescriptionGoat polyclonal antibody anti-MID2/TRIM1 (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications.
ApplicationsPep-ELISA/IHC
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Pig/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:4000.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 8µg/ml.
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolMID2
Gene ID11043
Uniprot IDTRIM1_HUMAN
Accession NumberNP_036348.2; NP_438112.2
Immunogen RegionC-Term
Immunogen SequenceQESPYVSGMKTCH
SpecificityThis antibody is expected to recognise both human isoforms of this protein.

Additional Info

Post Translational Modifications Phosphorylated on serine and threonine residues.
Function E3 ubiquitin ligase that plays a role in microtubule stabilization. Mediates the 'Lys-48'-linked polyubiquitination of LRRK2 to drive its localization to microtubules and its proteasomal degradation in neurons. This ubiquitination inhibits LRRK2 kinase activation by RAB29.
Protein Name Probable E3 Ubiquitin-Protein Ligase Mid2
Midin-2
Midline Defect 2
Midline-2
Ring Finger Protein 60
Ring-Type E3 Ubiquitin Transferase Mid2
Tripartite Motif-Containing Protein 1
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule-Associated
Alternative Antibody Names Anti-Probable E3 Ubiquitin-Protein Ligase Mid2 antibody
Anti-Midin-2 antibody
Anti-Midline Defect 2 antibody
Anti-Midline-2 antibody
Anti-Ring Finger Protein 60 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Mid2 antibody
Anti-Tripartite Motif-Containing Protein 1 antibody
Anti-MID2 antibody
Anti-FXY2 antibody
Anti-RNF60 antibody
Anti-TRIM1 antibody

Information sourced from Uniprot.org

Citations

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