Anti-MID1 antibody (71-120 aa) (STJ94125)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TRI18 at the amino acid range 71-120
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase Midline-1 (71-120 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MID1 |
| Gene ID | 4281 |
| Uniprot ID | TRI18_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TRI18 at the amino acid range 71-120 |
| Immunogen Region | 71-120 aa |
| Specificity | Midline-1 Polyclonal Antibody detects endogenous levels of Midline-1 protein. |
Additional Info
| Post Translational Modifications | Phosphorylated on serine and threonine residues. |
| Function | Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. |
| Protein Name | E3 Ubiquitin-Protein Ligase Midline-1MidinPutative Transcription Factor XprfRing Finger Protein 59Ring Finger Protein Midline-1Ring-Type E3 Ubiquitin Transferase Midline-1Tripartite Motif-Containing Protein 18 |
| Database Links | Reactome: R-HSA-877300 |
| Cellular Localisation | CytoplasmCytoskeletonSpindleMicrotubule-AssociatedIt Is Associated With Microtubules Throughout The Cell CycleCo-Localizing With Cytoplasmic Fibers In Interphase And With The Mitotic Spindle And Midbodies During Mitosis And Cytokinesis |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Midline-1 antibodyAnti-Midin antibodyAnti-Putative Transcription Factor Xprf antibodyAnti-Ring Finger Protein 59 antibodyAnti-Ring Finger Protein Midline-1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Midline-1 antibodyAnti-Tripartite Motif-Containing Protein 18 antibodyAnti-MID1 antibodyAnti-FXY antibodyAnti-RNF59 antibodyAnti-TRIM18 antibodyAnti-XPRF antibody |
Information sourced from Uniprot.org