• Western blot analysis of lysates from 293 cells, using TRI18 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRI18 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293 cells using Midline-1 Polyclonal Antibody
  • Immunofluorescence analysis of HeLa cells, using TRI18 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Midline-1 Polyclonal Antibody

Anti-MID1 antibody (71-120 aa) (STJ94125)

SKU:
STJ94125

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase Midline-1 (71-120 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MID1
Gene ID: 4281
Uniprot ID: TRI18_HUMAN
Immunogen Region: 71-120 aa
Specificity: Midline-1 Polyclonal Antibody detects endogenous levels of Midline-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human TRI18 at the amino acid range 71-120
Post Translational Modifications Phosphorylated on serine and threonine residues.
Function Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
Protein Name E3 Ubiquitin-Protein Ligase Midline-1
Midin
Putative Transcription Factor Xprf
Ring Finger Protein 59
Ring Finger Protein Midline-1
Ring-Type E3 Ubiquitin Transferase Midline-1
Tripartite Motif-Containing Protein 18
Database Links Reactome: R-HSA-877300
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Microtubule-Associated
It Is Associated With Microtubules Throughout The Cell Cycle
Co-Localizing With Cytoplasmic Fibers In Interphase And With The Mitotic Spindle And Midbodies During Mitosis And Cytokinesis
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Midline-1 antibody
Anti-Midin antibody
Anti-Putative Transcription Factor Xprf antibody
Anti-Ring Finger Protein 59 antibody
Anti-Ring Finger Protein Midline-1 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Midline-1 antibody
Anti-Tripartite Motif-Containing Protein 18 antibody
Anti-MID1 antibody
Anti-FXY antibody
Anti-RNF59 antibody
Anti-TRIM18 antibody
Anti-XPRF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance