• Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Western blot analysis of 293t cells using Antibody diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-MIB1 antibody (901-950 aa) (STJ98646)

SKU:
STJ98646

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase MIB1 (901-950 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 100-300
WB 1:500-2000
ELISA 1:10000-20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MIB1
Gene ID: 57534
Uniprot ID: MIB1_HUMAN
Immunogen Region: 901-950 aa
Specificity: The antibody detects endogenous MIB1 protein
Immunogen: Synthetic peptide from the human protein at the amino acid range 901-950
Post Translational Modifications Ubiquitinated.possibly via autoubiquitination. Ubiquitinated.this modification is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock.
Function E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.
Protein Name E3 Ubiquitin-Protein Ligase Mib1
Dapk-Interacting Protein 1
Dip-1
Mind Bomb Homolog 1
Ring-Type E3 Ubiquitin Transferase Mib1
Zinc Finger Zz Type With Ankyrin Repeat Domain Protein 2
Database Links Reactome: R-HSA-2122948
Reactome: R-HSA-2644606
Reactome: R-HSA-2691232
Reactome: R-HSA-2894862
Reactome: R-HSA-2979096
Reactome: R-HSA-9013507
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriolar Satellite
Cell Membrane
Localizes To The Plasma Membrane
It Is Mitochondrial
However Such Localization Remains Unclear
Displaced From Centriolar Satellites In Response To Cellular Stress
Such As Ultraviolet Light (Uv) Radiation Or Heat Shock
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Mib1 antibody
Anti-Dapk-Interacting Protein 1 antibody
Anti-Dip-1 antibody
Anti-Mind Bomb Homolog 1 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Mib1 antibody
Anti-Zinc Finger Zz Type With Ankyrin Repeat Domain Protein 2 antibody
Anti-MIB1 antibody
Anti-DIP1 antibody
Anti-KIAA1323 antibody
Anti-ZZANK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance