• Western blot analysis of extracts of various cell lines, using MIB1/DIP1 antibody (STJ111320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using MIB1/DIP1 antibody (STJ111320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MIB1 antibody (560-860) (STJ111320)

SKU:
STJ111320

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MIB1 (560-860) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MIB1
Gene ID: 57534
Uniprot ID: MIB1_HUMAN
Immunogen Region: 560-860
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 560-860 of human MIB1/DIP1 (NP_065825.1).
Immunogen Sequence: DSEGDTPLHDAISKKRDDIL AVLLEAGADVTITNNNGFNA LHHAALRGNPSAMRVLLSKL PRPWIVDEKKDDGYTALHLA ALNNHVEVAELLVHQGNANL DIQNVNQQTALHLAVERQHT QIVRLLVRAGAKLDIQDKDG DTPLHEALRHHTLSQLRQLQ DMQDVGKVDAAWEPSKNTLI MGLGTQGAEKKSAASIACFL AANGADLSIRNKKGQSPLDL CPDPNLCKALAKCHKEKVS
Tissue Specificity Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
Post Translational Modifications Ubiquitinated.possibly via autoubiquitination. Ubiquitinated.this modification is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock.
Function E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.
Protein Name E3 Ubiquitin-Protein Ligase Mib1
Dapk-Interacting Protein 1
Dip-1
Mind Bomb Homolog 1
Ring-Type E3 Ubiquitin Transferase Mib1
Zinc Finger Zz Type With Ankyrin Repeat Domain Protein 2
Database Links Reactome: R-HSA-2122948
Reactome: R-HSA-2644606
Reactome: R-HSA-2691232
Reactome: R-HSA-2894862
Reactome: R-HSA-2979096
Reactome: R-HSA-9013507
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriolar Satellite
Cell Membrane
Localizes To The Plasma Membrane
It Is Mitochondrial
However Such Localization Remains Unclear
Displaced From Centriolar Satellites In Response To Cellular Stress
Such As Ultraviolet Light (Uv) Radiation Or Heat Shock
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Mib1 antibody
Anti-Dapk-Interacting Protein 1 antibody
Anti-Dip-1 antibody
Anti-Mind Bomb Homolog 1 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Mib1 antibody
Anti-Zinc Finger Zz Type With Ankyrin Repeat Domain Protein 2 antibody
Anti-MIB1 antibody
Anti-DIP1 antibody
Anti-KIAA1323 antibody
Anti-ZZANK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance