![Anti-MHC II DR-alpha + beta chain antibody [Bra30] (STJ16101040)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149756/302501/STJ16101040_1__44307.1646940671.png?c=1 "Anti-MHC II DR-alpha + beta chain antibody [Bra30] (STJ16101040)")
![Anti-MHC II DR-alpha + beta chain antibody [Bra30] (STJ16101040)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/149756/302501/STJ16101040_1__44307.1646940671.png?c=1 "Anti-MHC II DR-alpha + beta chain antibody [Bra30] (STJ16101040)")
| Short Description : | Mouse monoclonal anti-MHC II DR-alpha + beta chain for use in FC and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | FC/IHC |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | Bra30 |
| Isotype: | IgG2ak |
| Conjugation: | Unconjugated |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification: | Affinity purified from tissue culture. |
| Dilution Range: | Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 mi |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Immunogen: | A BALB/c mouse was immunized with REH (human ALL cell line). Fusion partner: SP-2/0. |
| Background | MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an Alpha and Beta chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and-DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. |
Information sourced from Uniprot.org
![Anti-MHC II DP+DR-beta chain antibody [Bra14] (STJ16101036)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/149752/302497/STJ16101036_1__28984.1646940669.jpg?c=1 "Anti-MHC II DP+DR-beta chain antibody [Bra14] (STJ16101036)")
