• Western blot analysis of extracts of various cell lines, using MGMT antibody (STJ24549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MGMT antibody (1-238) (STJ24549)

SKU:
STJ24549

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MGMT (1-238) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MGMT
Gene ID: 4255
Uniprot ID: MGMT_HUMAN
Immunogen Region: 1-238
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of human MGMT (NP_002403.2).
Immunogen Sequence: MLGQPAPLERFASRRPQVLA VRTVCDLVLGKMDKDCEMKR TTLDSPLGKLELSGCEQGLH EIKLLGKGTSAADAVEVPAP AAVLGGPEPLMQCTAWLNAY FHQPEAIEEFPVPALHHPVF QQESFTRQVLWKLLKVVKFG EVISYQQLAALAGNPKAARA VGGAMRGNPVPILIPCHRVV CSSGAVGNYSGGLAVKEWLL AHEGHRL
Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Protein Name Methylated-Dna--Protein-Cysteine Methyltransferase
6-O-Methylguanine-Dna Methyltransferase
Mgmt
O-6-Methylguanine-Dna-Alkyltransferase
Database Links Reactome: R-HSA-5657655
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Methylated-Dna--Protein-Cysteine Methyltransferase antibody
Anti-6-O-Methylguanine-Dna Methyltransferase antibody
Anti-Mgmt antibody
Anti-O-6-Methylguanine-Dna-Alkyltransferase antibody
Anti-MGMT antibody

Information sourced from Uniprot.org

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