Anti-MGMT antibody (1-238) (STJ24549)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MGMT (1-238) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MGMT |
| Gene ID | 4255 |
| Uniprot ID | MGMT_HUMAN |
| Immunogen Region | 1-238 |
| Immunogen Sequence | MLGQPAPLERFASRRPQVLA VRTVCDLVLGKMDKDCEMKR TTLDSPLGKLELSGCEQGLH EIKLLGKGTSAADAVEVPAP AAVLGGPEPLMQCTAWLNAY FHQPEAIEEFPVPALHHPVF QQESFTRQVLWKLLKVVKFG EVISYQQLAALAGNPKAARA VGGAMRGNPVPILIPCHRVV CSSGAVGNYSGGLAVKEWLL AHEGHRL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (NP_002403.2). |
Additional Info
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Protein Name | Methylated-Dna--Protein-Cysteine Methyltransferase6-O-Methylguanine-Dna MethyltransferaseMgmtO-6-Methylguanine-Dna-Alkyltransferase |
| Database Links | Reactome: R-HSA-5657655 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Methylated-Dna--Protein-Cysteine Methyltransferase antibodyAnti-6-O-Methylguanine-Dna Methyltransferase antibodyAnti-Mgmt antibodyAnti-O-6-Methylguanine-Dna-Alkyltransferase antibodyAnti-MGMT antibody |
Information sourced from Uniprot.org