• Western blot analysis of extracts of various cell lines, using MGLL antibody (STJ28737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of mouse heart, using MGLL antibody (STJ28737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded rat ovary using MGLL rabbit polyclonal antibody (STJ28737) at dilution of 1:50 (40x lens).
  • Immunohistochemistry of paraffin-embedded human liver using MGLL rabbit polyclonal antibody (STJ28737) at dilution of 1:50 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse heart using MGLL rabbit polyclonal antibody (STJ28737) at dilution of 1:50 (40x lens).

Anti-MGLL antibody (1-303) (STJ28737)

SKU:
STJ28737

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Host: Rabbit
Applications: WB, IHC
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-MGLL (1-303) is suitable for use in Western Blot and Immunohistochemistry.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: MGLL
Gene ID: 11343
Uniprot ID: MGLL_HUMAN
Immunogen Region: 1-303
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human MGLL (NP_009214.1).
Tissue Specificity Detected in adipose tissue, lung, liver, kidney, brain and heart.
Function Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
Protein Name Monoglyceride Lipase
Mgl
Hu-K5
Lysophospholipase Homolog
Lysophospholipase-Like
Monoacylglycerol Lipase
Magl
Database Links Reactome: R-HSA-1482883
Reactome: R-HSA-163560
Reactome: R-HSA-426048
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Monoglyceride Lipase antibody
Anti-Mgl antibody
Anti-Hu-K5 antibody
Anti-Lysophospholipase Homolog antibody
Anti-Lysophospholipase-Like antibody
Anti-Monoacylglycerol Lipase antibody
Anti-Magl antibody
Anti-MGLL antibody

Information sourced from Uniprot.org

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