• Western blot analysis of extracts of various cell lines, using MGAT2 antibody (STJ111582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using MGAT2 antibody (STJ111582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MGAT2 antibody (178-447) (STJ111582)

SKU:
STJ111582

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MGAT2 (178-447) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MGAT2
Gene ID: 4247
Uniprot ID: MGAT2_HUMAN
Immunogen Region: 178-447
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 178-447 of human MGAT2 (NP_002399.1).
Immunogen Sequence: PFSIQLYPNEFPGSDPRDCP RDLPKNAALKLGCINAEYPD SFGHYREAKFSQTKHHWWWK LHFVWERVKILRDYAGLILF LEEDHYLAPDFYHVFKKMWK LKQQECPECDVLSLGTYSAS RSFYGMADKVDVKTWKSTEH NMGLALTRNAYQKLIECTDT FCTYDDYNWDWTLQYLTVSC LPKFWKVLVPQIPRIFHAGD CGMHHKKTCRPSTQSAQIES LLNNNKQYMFPETLTISEK
Function Plays an essential role in protein N-glycosylation. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) onto the free terminal mannose moiety in the core structure of the nascent N-linked glycan chain, giving rise to the second branch in complex glycans.
Protein Name Alpha-1 -6-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase
Beta-1 -2-N-Acetylglucosaminyltransferase Ii
Glcnac-T Ii
Gnt-Ii
Mannoside Acetylglucosaminyltransferase 2
N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase Ii
Database Links Reactome: R-HSA-4793952
Reactome: R-HSA-9694548
Reactome: R-HSA-975578
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Alpha-1 -6-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase antibody
Anti-Beta-1 -2-N-Acetylglucosaminyltransferase Ii antibody
Anti-Glcnac-T Ii antibody
Anti-Gnt-Ii antibody
Anti-Mannoside Acetylglucosaminyltransferase 2 antibody
Anti-N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase Ii antibody
Anti-MGAT2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance