| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MFGE8 (1-364) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MFGE8 |
| Gene ID: |
4240 |
| Uniprot ID: |
MFGM_HUMAN |
| Immunogen Region: |
1-364 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-364 of human MFGE8 (NP_005919.2). |
| Immunogen Sequence: |
LDICSKNPCHNGGLCEEISQ EVRGDVFPSYTCTCLKGYAG NHCETKCVEPLGLENGNIAN SQIAASSVRVTFLGLQHWVP ELARLNRAGMVNAWTPSSND DNPWIQVNLLRRMWVTGVVT QGASRLASHEYLKAFKVAYS LNGHEFDFIHDVNKKHKEFV GNWNKNAVHVNLFETPVEAQ YVRLYPTSCHTACTLRFELL |
| Tissue Specificity | Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas. |
| Post Translational Modifications | Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. |
| Function | Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Medin: Main constituent of aortic medial amyloid. |
| Protein Name | LactadherinBreast Epithelial Antigen Ba46HmfgMfgmMilk Fat Globule-Egf Factor 8Mfg-E8Sed1 Cleaved Into - Lactadherin Short Form - Medin |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-8957275Reactome: R-HSA-977225 |
| Cellular Localisation | MembranePeripheral Membrane ProteinSecretedCytoplasmic VesicleSecretory VesicleAcrosome MembraneLocated In The Acrosomal Region Of Zona-Pellucida Bound Sperm |
| Alternative Antibody Names | Anti-Lactadherin antibodyAnti-Breast Epithelial Antigen Ba46 antibodyAnti-Hmfg antibodyAnti-Mfgm antibodyAnti-Milk Fat Globule-Egf Factor 8 antibodyAnti-Mfg-E8 antibodyAnti-Sed1 Cleaved Into - Lactadherin Short Form - Medin antibodyAnti-MFGE8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and FIBP knockout (KO) HeLa cells, using [KO Validated] FIBP Rabbit polyclonal antibody (STJ11100784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89720/358201/STJ11100784_1__82816.1713122521.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FIBP knockout (KO) HeLa cells, using [KO Validated] FIBP Rabbit polyclonal antibody (STJ11100784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
