| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-METTL16 (263-562) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
METTL16 |
| Gene ID: |
79066 |
| Uniprot ID: |
MET16_HUMAN |
| Immunogen Region: |
263-562 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human METTL16 (NP_076991.3). |
| Immunogen Sequence: |
RIQGVPKVTYTEFCQGRTMR WALAWSFYDDVTVPSPPSKR RKLEKPRKPITFVVLASVMK ELSLKASPLRSETAEGIVVV TTWIEKILTDLKVQHKRVPC GKEEVSLFLTAIENSWIHLR RKKRERVRQLREVPRAPEDV IQALEEKKPTPKESGNSQEL ARGPQERTPCGPALREGEAA AVEGPCPSQESLSQEENPEP TEDERSEEKGGVEVLESCQG SSNGAQDQEASEQFGSPVA |
| Function | RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure. Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, preventing recognition of their 3'-splice site by U2AF1/U2AF35, thereby inhibiting splicing and protein production of S-adenosylmethionine synthase. In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs. Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA. Specifically binds the 3'-end of the MALAT1 long non-coding RNA. |
| Protein Name | Rna N6-Adenosine-Methyltransferase Mettl16Methyltransferase 10 Domain-Containing ProteinMethyltransferase-Like Protein 16N6-Adenosine-Methyltransferase Mettl16U6 Small Nuclear RnaAdenine-(43-N(6-Methyltransferase |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Rna N6-Adenosine-Methyltransferase Mettl16 antibodyAnti-Methyltransferase 10 Domain-Containing Protein antibodyAnti-Methyltransferase-Like Protein 16 antibodyAnti-N6-Adenosine-Methyltransferase Mettl16 antibodyAnti-U6 Small Nuclear Rna antibodyAnti-Adenine-(43-N(6-Methyltransferase antibodyAnti-METTL16 antibodyAnti-METT10D antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
