Anti-METTL14 antibody (1-210) (STJ111272)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal METTL14 (1-210) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | METTL14 |
| Gene ID | 57721 |
| Uniprot ID | MET14_HUMAN |
| Immunogen Region | 1-210 |
| Immunogen Sequence | MDSRLQEIRERQKLRRQLLA QQLGAESADSIGAVLNSKDE QREIAETRETCRASYDTSAP NAKRKYLDEGETDEDKMEEY KDELEMQQDEENLPYEEEIY KDSSTFLKGTQSLNPHNDYC QHFVDTGHRPQNFIRDVGLA DRFEEYPKLRELIRLKDELI AKSNTPPMYLQADIEAFDIR ELTPKFDVILLEPPLEEYYR ETGITANEKC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1). |
Additional Info
| Function | The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-AGGAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells. |
| Protein Name | N(6-Adenosine-Methyltransferase Non-Catalytic Subunit Mettl14Methyltransferase-Like Protein 14Hmettl14 |
| Database Links | Reactome: R-HSA-72203 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-N(6-Adenosine-Methyltransferase Non-Catalytic Subunit Mettl14 antibodyAnti-Methyltransferase-Like Protein 14 antibodyAnti-Hmettl14 antibodyAnti-METTL14 antibodyAnti-KIAA1627 antibody |
Information sourced from Uniprot.org