Anti-METTL1 antibody (STJA0006877)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human METTL1
General Information
| Short Description | Rabbit polyclonal anti-tRNA--methyltransferase--methyltransferase--methyltransferase-N7-methyltransferase-methyltransferase for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | METTL1 |
| Gene ID | 4234 |
| Uniprot ID | TRMB_HUMAN |
| Immunogen | Synthesized peptide derived from human METTL1 |
| Specificity | This antibody detects endogenous levels of METTL1 at Human, Mouse |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-27 by PKB/AKT1 inactivates its methyltransferase activity via a steric interference mechanism in the active site that locally disrupts the catalytic center. Phosphorylation at Ser-27 does not affect the interaction with WDR4. |
| Function | Catalytic component of METTL1-WDR4 methyltransferase complex that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs. Internal N(7)-methylguanine methylation of mRNAs in response to stress promotes their relocalization to stress granules, thereby suppressing their translation. Also methylates a specific subset of miRNAs, such as let-7. N(7)-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA). Acts as a regulator of embryonic stem cell self-renewal and differentiation. |
| Protein Name | TrnaGuanine-N(7--MethyltransferaseMethyltransferase-Like Protein 1MrnaGuanine-N(7--MethyltransferaseMirnaGuanine-N(7--MethyltransferaseTrnaGuanine(46-N(7-MethyltransferaseTrna(M7g46-Methyltransferase |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Guanine-N(7--Methyltransferase antibodyAnti-Methyltransferase-Like Protein 1 antibodyAnti-Mrna antibodyAnti-Guanine-N(7--Methyltransferase antibodyAnti-Mirna antibodyAnti-Guanine-N(7--Methyltransferase antibodyAnti-Trna antibodyAnti-Guanine(46-N(7-Methyltransferase antibodyAnti-Trna(M7g46-Methyltransferase antibodyAnti-METTL1 antibodyAnti-C12orf1 antibody |
Information sourced from Uniprot.org