| Short Description : | Mouse monoclonal anti-Methyl-CpG binding protein 2 (Full-Length) for use in IHC-F and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | IHC-F/WB |
| Host: | Mouse |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 4F11 |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Purification: | Affinity-purified |
| Dilution Range: | WB 1:5000-1:10000IHC 1:1000-1:5000 |
| Formulation: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide. |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquo |
| Gene Symbol: | MECP2 |
| Gene ID: | 4204 |
| Uniprot ID: | MECP2_HUMAN |
| Immunogen: | Full-length recombinant human protein |
| Immunogen Region: | Full-Length |
| Immunogen Sequence: | Human |
| Specificity: | Reacts with human, mouse, rat. |
| Tissue Specificity | Present in all adult somatic tissues tested. |
| Post Translational Modifications | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
| Protein Name | Methyl-Cpg-Binding Protein 2Mecp-2 ProteinMecp2 |
| Database Links | Reactome: R-HSA-8986944Reactome: R-HSA-9022534Reactome: R-HSA-9022535Reactome: R-HSA-9022537Reactome: R-HSA-9022538Reactome: R-HSA-9022692Reactome: R-HSA-9022699Reactome: R-HSA-9022702Reactome: R-HSA-9022707Reactome: R-HSA-9022927Reactome: R-HSA-9725371 |
| Cellular Localisation | NucleusColocalized With Methyl-Cpg In The GenomeColocalized With Tbl1x To The Heterochromatin Foci |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Protein 2 antibodyAnti-Mecp-2 Protein antibodyAnti-Mecp2 antibodyAnti-MECP2 antibody |
Information sourced from Uniprot.org
