Anti-Methyl-CpG-binding protein 2 antibody (C-Term) (STJA0040987)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide (REEPVDSRTPVTERVS, aa: 471-486) of C-terminus of human protein.
General Information
| Short Description | Rabbit polyclonal anti-Methyl-CpG-binding protein 2 (C-Term) for use in IHC-F and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purified |
| Dilution Range | WB 1:1000-1:5000IHC 0.73611111111111116 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at-20°C for long-term |
Target Information
| Gene Symbol | MECP2 |
| Gene ID | 4204 |
| Uniprot ID | MECP2_HUMAN |
| Immunogen | A synthetic peptide (REEPVDSRTPVTERVS, aa: 471-486) of C-terminus of human protein. |
| Immunogen Region | C-Term |
| Immunogen Sequence | Human |
| Specificity | Reacts with human, mouse, rat. |
Additional Info
| Tissue Specificity | Present in all adult somatic tissues tested. |
| Post Translational Modifications | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
| Protein Name | Methyl-Cpg-Binding Protein 2Mecp-2 ProteinMecp2 |
| Database Links | Reactome: R-HSA-8986944Reactome: R-HSA-9022534Reactome: R-HSA-9022535Reactome: R-HSA-9022537Reactome: R-HSA-9022538Reactome: R-HSA-9022692Reactome: R-HSA-9022699Reactome: R-HSA-9022702Reactome: R-HSA-9022707Reactome: R-HSA-9022927Reactome: R-HSA-9725371 |
| Cellular Localisation | NucleusColocalized With Methyl-Cpg In The GenomeColocalized With Tbl1x To The Heterochromatin Foci |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Protein 2 antibodyAnti-Mecp-2 Protein antibodyAnti-Mecp2 antibodyAnti-MECP2 antibody |
Information sourced from Uniprot.org