• Western blot analysis of extracts of various cell lines, using Mesothelin antibody (STJ11103653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Confocal imaging of paraffin-embedded Rat lung using Mesothelin Rabbit monoclonal antibody (STJ11103653, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of rat lung using Mesothelin rabbit monoclonal antibody (STJ11103653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Mesothelin Rabbit monoclonal antibody (STJ11103653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Mesothelin antibody (500-600) [S3MR] (STJ11103653)

SKU:
STJ11103653

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Mesothelin (500-600) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSLN
Gene ID: 10232
Uniprot ID: MSLN_HUMAN
Immunogen Region: 500-600
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Mesothelin (Q13421).
Immunogen Sequence: YFVKIQSFLGGAPTEDLKAL SQQNVSMDLATFMKLRTDAV LPLTVAEVQKLLGPHVEGLK AEERHRPVRDWILRQRQDDL DTLGLGLQGGIPNGYLVLDL S
Tissue Specificity Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
Post Translational Modifications Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
Function Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Protein Name Mesothelin
Cak1 Antigen
Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor
Mpf - Mesothelin - Cleaved Form
Database Links Reactome: R-HSA-163125
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Golgi Apparatus
Megakaryocyte-Potentiating Factor: Secreted
Isoform 3: Secreted
Alternative Antibody Names Anti-Mesothelin antibody
Anti-Cak1 Antigen antibody
Anti-Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor antibody
Anti-Mpf - Mesothelin - Cleaved Form antibody
Anti-MSLN antibody
Anti-MPF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance