• Western blot analysis of extracts of mouse spleen, using MERTK antibody (STJ27396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of HepG2 cells, using MERTK antibody (STJ27396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using MERTK rabbit polyclonal antibody (STJ27396) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS using MERTK antibody (STJ27396) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MERTK antibody (26-999) (STJ27396)

SKU:
STJ27396

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MERTK (26-999) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MERTK
Gene ID: 10461
Uniprot ID: MERTK_HUMAN
Immunogen Region: 26-999
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-999 of human MERTK (NP_006334.2).
Immunogen Sequence: REEAKPYPLFPGPFPGSLQT DHTPLLSLPHASGYQPALMF SPTQPGRPHTGNVAIPQVTS VESKPLPPLAFKHTVGHIIL SEHKGVKFNCSISVPNIYQD TTISWWKDGKELLGAHHAIT QFYPDDEVTAIIASFSITSV QRSDNGSYICKMKINNEEIV SDPIYIEVQGLPHFTKQPES MNVTRNTAFNLTCQAVGPPE PVNIFWVQNSSRVNEQPEKS PSVLTVPGLTEMAVFSCEA
Tissue Specificity Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.
Post Translational Modifications Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2.
Function Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Protein Name Tyrosine-Protein Kinase Mer
Proto-Oncogene C-Mer
Receptor Tyrosine Kinase Mertk
Database Links Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Mer antibody
Anti-Proto-Oncogene C-Mer antibody
Anti-Receptor Tyrosine Kinase Mertk antibody
Anti-MERTK antibody
Anti-MER antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance