Anti-MERS-CoV Spike RBD antibody (18-725) (STJ11103388)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MERS-CoV Spike RBD (18-725) antibody for use in WB, IF, ICC and ELISA in mers-cov samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | MERS-CoV |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen Region | 18-725 |
| Immunogen Sequence | YVDVGPDSVKSACIEVDIQQ TFFDKTWPRPIDVSKADGII YPQGRTYSNITITYQGLFPY QGDHGDMYVYSAGHATGTTP QKLFVANYSQDVKQFANGFV VRIGAAANSTGTVIISPSTS ATIRKIYPAFMLGSSVGNFS DGKMGRFFNHTLVLLPDGCG TLLRAFYCILEPRSGNHCPA GNSYTSFATYHTPATDCSDG NYNRNASLNSFKEYFNLRNC TFMYTYNITEDEILEWFGI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 18-725 of coronavirus Spike RBD (YP_009047204.1). |
Additional Info
| Background | It s been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD) , which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Information sourced from Uniprot.org