• Western blot analysis of extracts of various cell lines, using MEMO1 antibody (STJ111315) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using MEMO1 antibody (STJ111315) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human prostate using MEMO1 antibody (STJ111315) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human prostate using MEMO1 antibody (STJ111315) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using MEMO1 antibody (STJ111315) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using MEMO1 antibody (STJ111315) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-MEMO1 antibody (1-297) (STJ111315)

SKU:
STJ111315

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MEMO1 (1-297) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MEMO1
Gene ID: 51072
Uniprot ID: MEMO1_HUMAN
Immunogen Region: 1-297
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human MEMO1 (NP_057039.1).
Immunogen Sequence: MSNRVVCREASHAGSWYTAS GPQLNAQLEGWLSQVQSTKR PARAIIAPHAGYTYCGSCAA HAYKQVDPSITRRIFILGPS HHVPLSRCALSSVDIYRTPL YDLRIDQKIYGELWKTGMFE RMSLQTDEDEHSIEMHLPYT AKAMESHKDEFTIIPVLVGA LSESKEQEFGKLFSKYLADP SNLFVVSSDFCHWGQRFRYS YYDESQGEIYRSIEHLDKMG MSIIEQLDPVSFSNYLKKY
Function May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration.
Protein Name Protein Memo1
C21orf19-Like Protein
Hepatitis C Virus Ns5a-Transactivated Protein 7
Hcv Ns5a-Transactivated Protein 7
Mediator Of Erbb2-Driven Cell Motility 1
Mediator Of Cell Motility 1
Memo-1
Database Links Reactome: R-HSA-6785631
Alternative Antibody Names Anti-Protein Memo1 antibody
Anti-C21orf19-Like Protein antibody
Anti-Hepatitis C Virus Ns5a-Transactivated Protein 7 antibody
Anti-Hcv Ns5a-Transactivated Protein 7 antibody
Anti-Mediator Of Erbb2-Driven Cell Motility 1 antibody
Anti-Mediator Of Cell Motility 1 antibody
Anti-Memo-1 antibody
Anti-MEMO1 antibody
Anti-C2orf4 antibody
Anti-MEMO antibody
Anti-NS5ATP7 antibody
Anti-CGI-27 antibody

Information sourced from Uniprot.org

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