• Western blot analysis of extracts of various cell lines, using MELK Rabbit monoclonal antibody (STJ11101891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-MELK antibody (1-100) [S1MR] (STJ11101891)

SKU:
STJ11101891

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MELK (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MELK
Gene ID: 9833
Uniprot ID: MELK_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MELK (NP_055606.1).
Immunogen Sequence: MKDYDELLKYYELHETIGTG GFAKVKLACHILTGEMVAIK IMDKNTLGSDLPRIKTEIEA LKNLRHQHICQLYHVLETAN KIFMVLEYCPGGELFDYIIS
Tissue Specificity Expressed in placenta, kidney, thymus, testis, ovary and intestine.
Post Translational Modifications Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8 (Probable).
Function Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity.phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
Protein Name Maternal Embryonic Leucine Zipper Kinase
Hmelk
Protein Kinase Eg3
Peg3 Kinase
Protein Kinase Pk38
Hpk38
Tyrosine-Protein Kinase Melk
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Maternal Embryonic Leucine Zipper Kinase antibody
Anti-Hmelk antibody
Anti-Protein Kinase Eg3 antibody
Anti-Peg3 Kinase antibody
Anti-Protein Kinase Pk38 antibody
Anti-Hpk38 antibody
Anti-Tyrosine-Protein Kinase Melk antibody
Anti-MELK antibody
Anti-KIAA0175 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance