Anti-MEL18/PCGF2 antibody (C-Term) (STJ70369)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-MEL18/PCGF2 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 37-38 kDa band observed in lysates of cell lines A549 and PD19 (calculated MW of 37.8kDa according to NP_009075.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 h |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PCGF2 |
| Gene ID | 7703 |
| Uniprot ID | PCGF2_HUMAN |
| Accession Number | NP_009075.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | KMTVNGAPVPPLT |
Additional Info
| Post Translational Modifications | Phosphorylated. Homodimer formation is regulated by phosphorylation with only unphosphorylated proteins forming homodimers. |
| Function | Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity. |
| Protein Name | Polycomb Group Ring Finger Protein 2Dna-Binding Protein Mel-18Ring Finger Protein 110Zinc Finger Protein 144 |
| Database Links | Reactome: R-HSA-3108214Reactome: R-HSA-3899300Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4655427Reactome: R-HSA-5617472Reactome: R-HSA-8953750 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Polycomb Group Ring Finger Protein 2 antibodyAnti-Dna-Binding Protein Mel-18 antibodyAnti-Ring Finger Protein 110 antibodyAnti-Zinc Finger Protein 144 antibodyAnti-PCGF2 antibodyAnti-MEL18 antibodyAnti-RNF110 antibodyAnti-ZNF144 antibody |
Information sourced from Uniprot.org