• Immunohistochemistry analysis of paraffin-embedded mouse brain using MEKK2 Rabbit monoclonal antibody (STJ11103104) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using MEKK2 Rabbit monoclonal antibody (STJ11103104) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MEKK2 Rabbit monoclonal antibody (STJ11103104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-MEKK2 antibody (1-100) [S4MR] (STJ11103104)

SKU:
STJ11103104

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MEKK2 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP3K2
Gene ID: 10746
Uniprot ID: M3K2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEKK2 (Q9Y2U5).
Immunogen Sequence: MDDQQALNSIMQDLAVLHKA SRPALSLQETRKAKSSSPKK QNDVRVKFEHRGEKRILQFP RPVKLEDLRSKAKIAFGQSM DLHYTNNELVIPLTTQDDLD
Post Translational Modifications Autophosphorylated. Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation.
Function Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7. Plays a role in caveolae kiss-and-run dynamics.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase 2
Mapk/Erk Kinase Kinase 2
Mek Kinase 2
Mekk 2
Cellular Localisation Cytoplasm
Nucleus
Upon Egf Stimulation
Translocates Into The Nucleus
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase 2 antibody
Anti-Mapk/Erk Kinase Kinase 2 antibody
Anti-Mek Kinase 2 antibody
Anti-Mekk 2 antibody
Anti-MAP3K2 antibody
Anti-MAPKKK2 antibody
Anti-MEKK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance