| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MEF2D (431-521) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MEF2D |
| Gene ID: |
4209 |
| Uniprot ID: |
MEF2D_HUMAN |
| Immunogen Region: |
431-521 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 431-521 of human MEF2D (NP_005911.1). |
| Immunogen Sequence: |
TTHPHISIKSEPVSPSRERS PAPPPPAVFPAARPEPGDGL SSPAGGSYETGDRDDGRGDF GPTLGLLRPAPEPEAEGSAV KRMRLDTWTLK |
| Post Translational Modifications | Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phosphorylation on Ser-180 can be enhanced by EGF. Phosphorylated and activated by CaMK4. Acetylated on Lys-439 by CREBBP. Acetylated by EP300. Deacetylated by SIRT1 and HDAC3. Sumoylated on Lys-439 with SUMO2 but not SUMO1.which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3. Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. |
| Function | Transcriptional activator which binds specifically to the MEF2 element, 5'-YTAAT(4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis. |
| Protein Name | Myocyte-Specific Enhancer Factor 2d |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-400253Reactome: R-HSA-525793Reactome: R-HSA-9031628Reactome: R-HSA-9707616 |
| Cellular Localisation | NucleusTranslocated By Hdac4 To Nuclear Dots |
| Alternative Antibody Names | Anti-Myocyte-Specific Enhancer Factor 2d antibodyAnti-MEF2D antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
